The 14-3-3 beta/zeta Monoclonal Antibody (CAB9152) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in applications such as Western blotting.The 14-3-3 beta/zeta protein is a key player in various cellular processes, including cell cycle control, apoptosis, and signal transduction. Its involvement in these pathways makes it a valuable target for studying diseases related to cell dysfunction and signaling dysregulation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
14-3-3 beta/zeta Monoclonal Antibody
SKU:
CAB9152
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1454
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
HT-29, A-549, NIH/3T3, Jurkat, Mouse lung, Mouse kidney, Rat spleen
Cellular Localization:
Cytoplasm, Cytosol, Extracellular Exosome, Focal Adhesion, Melanosome, Perinuclear Region Of Cytoplasm.
Calculated MW:
28kDa
Observed MW:
28kDa
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Purification Method
Affinity purification
Gene ID
7529 7534
RRID
AB_2863674
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using 14-3-3 beta/zeta Rabbit mAb (CAB9152) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of NIH-3T3 cells using 14-3-3 beta/zeta Rabbit mAb (CAB9152) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
