Anti-14-3-3 gamma Antibody (CAB9162)

SKU:
CAB9162
€129 - €349

Description

Technical Manual

Anti-14-3-3 gamma Antibody

Product Name:Anti-14-3-3 gamma Antibody
Product SKU:CAB9162
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Immunogen:A synthesized peptide derived from human 14-3-3 gamma
Sequence:

Email for sequence

Gene ID:7532
Swiss Prot:P61981
Synonyms:14-3-3GAMMA; PPP1R170
Calculated MW:28kDa
Observed MW:28KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry
Positive Samples:HeLa, A-431, NIH/3T3, Jurkat, Mouse liver, Mouse heart, Rat lung
Cellular Location:

Product Images

Anti-14-3-3 gamma Antibody (CAB9162)

Western blot - 14-3-3 gamma Rabbit mAb (CAB9162)

Uniprot Information

UniProt Protein Function:14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane

Molecular Function:insulin-like growth factor receptor binding; protein binding; protein kinase C binding

Biological Process: G2/M transition of mitotic cell cycle; regulation of neuron differentiation; regulation of synaptic plasticity

NCBI Summary:This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
UniProt Code:P61981
NCBI GenInfo Identifier:48428721
NCBI Gene ID:7532
NCBI Accession:P61981.2
UniProt Secondary Accession:P61981,O70457, P35214, Q6FH52, Q9UDP2, Q9UN99,
UniProt Related Accession:P61981
Molecular Weight:28,303 Da
NCBI Full Name:14-3-3 protein gamma
NCBI Synonym Full Names:tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
NCBI Official Symbol:YWHAG
NCBI Official Synonym Symbols:PPP1R170; 14-3-3GAMMA
NCBI Protein Information:14-3-3 protein gamma
UniProt Protein Name:14-3-3 protein gamma
UniProt Synonym Protein Names:Protein kinase C inhibitor protein 1; KCIP-1
Protein Family:14-3-3 protein
UniProt Gene Name:YWHAG
UniProt Entry Name:1433G_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
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