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14-3-3 Theta Rabbit Monoclonal Antibody (CAB8936)

SKU:
CAB8936
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:14-3-3 Theta Rabbit Monoclonal Antibody
SKU:CAB8936
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 Theta (P27348).
Sequence:MEKT ELIQ KAKL AEQA ERYD DMAT CMKA VTEQ GAEL SNEE RNLL SVAY KNVV GGRR SAWR VISS IEQK TDTS DKKL QLIK DYRE KVES ELRS ICTT VLEL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:1C5; HS1; 14-3-3; 14-3-3 Theta
Positive Sample:C6,U-251MG,Mouse testis,Mouse brain,Rat testis
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:28kDa
Observed MW:28kDa

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Purification Method:Affinity purification
Gene ID:10971
Clone Number:ARC1358
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using 14-3-3 Theta Rabbit mAb (CAB8936) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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