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14-3-3 Theta Rabbit Polyclonal Antibody (CAB2563)

SKU:
CAB2563
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
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€139 - €419
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Description

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Product Name:14-3-3 Theta Rabbit Polyclonal Antibody
SKU:CAB2563
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 49-149 of human 14-3-3 Theta (NP_006817.1).
Sequence:KNVV GGRR SAWR VISS IEQK TDTS DKKL QLIK DYRE KVES ELRS ICTT VLEL LDKY LIAN ATNP ESKV FYLK MKGD YFRY LAEV ACGD DRKQ TIDN SQGA Y
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:1C5; HS1; 14-3-3; 14-3-3 Theta
Positive Sample:SW480,BT-474,HeLa,Jurkat,Mouse liver,Mouse kidney,Mouse brain
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:28kDa
Observed MW:32kDa

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Purification Method:Affinity purification
Gene ID:10971
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using 14-3-3 Theta antibody (CAB2563) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.

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