The MRP4/ABCC4 Antibody (CAB2198) is a high-quality antibody developed for reliable detection and analysis of target proteins. This cell surface transporter is involved in the efflux of various endogenous and xenobiotic substrates, playing a key role in drug resistance mechanisms.Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the ABCC4 protein, allowing for accurate detection and analysis in a variety of cell types.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRP4/ABCC4 Antibody
SKU:
CAB2198
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRP4, MOATB, MOAT-B, MRP4/ABCC4
Positive Sample:
HeLa, HeLa Cytoplasm extracts, 293T
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
150kDa
Observed MW:
140-200kDa
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10257
RRID
AB_2764215
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MRP4/ABCC4 Rabbit pAb (CAB2198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using MRP4/ABCC4 Rabbit pAb (CAB2198) at dilution of 1:150 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human oophoroma using MRP4/ABCC4 Rabbit pAb (CAB2198) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human prostate cancer using MRP4/ABCC4 Rabbit pAb (CAB2198) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
