Anti-MRP4/ABCC4 Antibody (CAB2198)

Antibody Name:	Anti-MRP4 / ABCC4 Antibody
Antibody SKU:	CAB2198
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 495-705 of human MRP4 / ABCC4 (NP_001098985.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 495-705 of human MRP4 / ABCC4 (NP_001098985.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGKK YEKE RYEK VIKA CALK KDLQ LLED GDLT VIGD RGTT LSGG QKAR VNLA RAVY QDAD IYLL DDPL SAVD AEVS RHLF ELCI CQIL HEKI TILV THQL QYLK AASQ ILIL KDGK MVQK GTYT EFLK SGID FGSL LKKD NEES EQPP VPGT PTLR NRTF SESS VWSQ QSSR PSLK DGAL ESQD TENV PVTL SEEN RSEG KVGF QAYK NYF
Gene ID:	10257
Uniprot:	O15439
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	88kDa/96kDa/144kDa/149kDa
Observed MW:	170kDa

Synonyms:

ABCC4, MOAT-B, MOATB, MRP4

Background:

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	ABCC4: a widely-expressed multi-pass membrane protein. An organic anion pump involved in cellular detoxification. Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
UniProt Protein Details:	Protein type:Transporter; Membrane protein, multi-pass; Transporter, ABC family; Membrane protein, integral Chromosomal Location of Human Ortholog: 13q32 Cellular Component: membrane; basolateral plasma membrane; integral to membrane; plasma membrane; platelet dense granule membrane Molecular Function:ATPase activity, coupled to transmembrane movement of substances; 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; ATP binding Biological Process: response to drug; platelet activation; platelet degranulation; positive regulation of smooth muscle cell proliferation; response to organic nitrogen; blood coagulation; transmembrane transport; response to organic cyclic substance
NCBI Summary:	The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
UniProt Code:	O15439
NCBI GenInfo Identifier:	206729914
NCBI Gene ID:	10257
NCBI Accession:	O15439.3
UniProt Related Accession:	O15439
Molecular Weight:
NCBI Full Name:	Multidrug resistance-associated protein 4
NCBI Synonym Full Names:	ATP binding cassette subfamily C member 4
NCBI Official Symbol:	ABCC4
NCBI Official Synonym Symbols:	MRP4; MOATB; MOAT-B
NCBI Protein Information:	multidrug resistance-associated protein 4
UniProt Protein Name:	Multidrug resistance-associated protein 4
UniProt Synonym Protein Names:	ATP-binding cassette sub-family C member 4; MRP/cMOAT-related ABC transporter; Multi-specific organic anion transporter B; MOAT-B
Protein Family:	ABC transporter C family
UniProt Gene Name:	ABCC4
UniProt Entry Name:	MRP4_HUMAN

	Western blot analysis of extracts of various cell lines, using MRP4 / ABCC4 antibody (CAB2198) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat testis using MRP4/ABCC4 antibody (CAB2198) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using MRP4/ABCC4 antibody (CAB2198) at dilution of 1:150 (40x lens).
	Immunofluorescence analysis of HeLa cells using MRP4 / ABCC4 antibody (CAB2198) at dilution of 1:100. Blue: DAPI for nuclear staining.

ELISA Kits

Human ABCC4 / MRP4 ELISA Kit

Human Multidrug resistance-associated protein 4 (ABCC4) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)