ABM (Abamectin) ELISA Kit (FSES0051)



Technical Manual

ABM (Abamectin) ELISA Kit

Kit Name: ABM (Abamectin) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Abamectin (ABM) in samples, such as milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, ABM in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-ABM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of ABM. The concentration of ABM in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.05 ppb (ng/mL)
Reaction Mode: 25°C,30 min~30 min~15 min
Detection Limit: Raw milk, Finished milk, Yogurt ---about 0.5 ppb
Cross Reactivity: Abamectin--- 100%, Ivermectin---75%, Doramectin---33%, Eprinomectin---100%.
Sample Recovery Rate: 100%±20%.
Kits Components:
Item Specifications
ELISA Microtiter Plate 96 wells
Standard Liquid 1 mL each (0 ppb, 0.05 ppb, 0.1 ppb, 0.2 ppb, 0.4 ppb, 0.8 ppb)
Antibody Working Solution 7 mL
HRP Conjugate 12 mL
Milk Precipitant 10 mL
Sample Diluent A 20 mL
Sample Diluent B 20 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20×Concentrated Wash Buffer 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Oscillators, Centrifuge, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Methanol.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
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