Description
cGMP ELISA Kit
The cGMP ELISA Kit (SKU: GXEL00004) is a high-sensitivity quantitative ELISA assay developed for the quantitative measurement of cGMP in Plasma, Serum. The assay delivers a detection range of 0.78-100 pmol/mL and a sensitivity of 0.78 pmol/mL and is read colorimetrically at 450 nm, making it well suited to quantitative research workflows.
<p>Guanosine 3’, 5’-cyclic monophosphate (cGMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cGMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cGMP ELISA Kit is based on the competition between HRP-labeled cGMP and free cGMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cGMP MAb) binding sites. Because the concentration of the HRP-labeled cGMP is held constant while the concentration of cGMP varies, the amount of HRP-labeled cGMP that is able to bind to the cGMP MAb will be inversely proportional to the concentration of cGMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50 (50% B/B0) of approximately 5 pmol/mL and a detection limit of approximately 0.1 pmol/mL.</p>
Supplied with pre-coated microplates and all core reagents, the cGMP ELISA Kit gives you a validated, ready-to-use solution for cGMP research. Get accurate and reliable results with the cGMP ELISA Kit from Assay Genie.
| Product Name: | cGMP ELISA Kit |
| SKU: | GXEL00004 |
| Accession: | CAS: 7665-99-8 |
| Assay Type: | Quantitative |
| Detection Method: | Colorimetric |
| Sensitivity: | 0.78 pmol/mL |
| Range: | 0.78-100 pmol/mL |
| Sample Type: | Plasma, Serum |
| Storage: | 2-8°C. See Storage & Stability for details. |
| Note: | For Research Use Only. Not for diagnostic procedures. |
| Parameter | Specification |
|---|---|
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Sensitivity | 0.78 pmol/mL |
| Detection Range | 0.78-100 pmol/mL |
| Recovery | 80-120% |
| Sample Types | Plasma, Serum |
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Store the kit at 2-8°C on receipt. Avoid repeated freeze-thaw cycles.
