The cGMP (Cyclic GMP) ELISA Kit from AssayGenie is a highly sensitive and specific assay designed for the accurate detection of cGMP levels in various samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for research applications in the fields of cardiovascular diseases, neurological disorders, and more.Cyclic GMP (cGMP) is a crucial signaling molecule involved in regulating various physiological processes, including smooth muscle relaxation, platelet aggregation, and neurotransmission. Dysregulation of cGMP levels has been linked to conditions such as hypertension, erectile dysfunction, and heart failure, highlighting the importance of monitoring and studying this biomarker.With the AssayGenie cGMP ELISA Kit, researchers can accurately quantify cGMP levels in their samples, aiding in the understanding of disease mechanisms and the development of potential therapeutic interventions. Trust AssayGenie for reliable and high-quality ELISA kits for your research needs.
Product Name:
cGMP (Cyclic GMP) ELISA Kit
SKU:
UNES00052
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.47 pmol/mL
Detection range:
0.78-50 pmol/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
