The ACADVL Antibody (CAB7865) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By targeting the ACADVL protein, researchers can detect and analyze its expression in various cell types, making it ideal for studies in metabolism, lipidomics, and mitochondrial disorders.ACADVL plays a crucial role in the breakdown of long-chain fatty acids for energy production, making it a key player in cellular metabolism. Dysregulation of ACADVL activity has been linked to metabolic disorders such as fatty acid oxidation disorders and mitochondrial dysfunction.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACADVL Antibody
SKU:
CAB7865
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACAD6, LCACD, VLCAD, ACADVL
Positive Sample:
K-562, LO2, A-549, U-87MG, HeLa, SW620, Mouse liver, Rat liver
Cellular Localization:
Mitochondrion Inner Membrane.
Calculated MW:
70kDa
Observed MW:
70kDa
The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
37
RRID
AB_2768196
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ACADVL Rabbit pAb (CAB7865) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using ACADVL Rabbit pAb (CAB7865) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
