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Anti-Acetyl-Histone H3-K23 Antibody (CAB18154)

SKU:
CAB18154
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Acetyl-Histone H3-K23 Antibody
Antibody SKU:CAB18154
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic peptide of human Acetyl-Histone H3-K23.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:HeLa, NIH/3T3
Immunogen:A synthetic peptide of human Acetyl-Histone H3-K23.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8350
Uniprot:P68431
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:17kDa
Western blot - Acetyl-Histone H3-K23 Rabbit pAb Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K23 antibody at 1:500 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K23 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb Immunohistochemistry of paraffin-embedded human tonsil using Acetyl-Histone H3-K23 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K23 at dilution of 1:100. Blue: DAPI for nuclear staining. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K23 at dilution of 1:100. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K23 at dilution of 1:100. Blue: DAPI for nuclear staining. U2OS cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation - Acetyl-Histone H3-K23 Rabbit pAb Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K23 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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