Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody (CAB7255)

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CAB7255
  • Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (CAB7255) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37? for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).
€99 - €319

Description

Technical Manual

Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody

Product Name:Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody
Product SKU:CAB7255
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen:A synthetic peptide of human Acetyl-Histone H3-K9
Sequence:

Email for sequence

Gene ID:8290
Swiss Prot:Q16695
Synonyms:H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW:15kDa
Observed MW:17KDa
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Application:WB IHC IF IP ChIP ChIPseq
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIPseq 1:20 - 1:50
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation 
Positive Samples:HeLa, NIH/3T3, C6
Cellular Location:Chromosome, Nucleus

Product Images

Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody (CAB7255)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (CAB7255) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37'C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody (CAB7255)

Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).

Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody (CAB7255)

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).

Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody (CAB7255)

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).

Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody (CAB7255)

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (CAB7255) at dilution of 1:200 (40x lens).

Uniprot Information

UniProt Protein Function:HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Code:Q16695
NCBI GenInfo Identifier:18202512
NCBI Gene ID:8290
NCBI Accession:Q16695.3
UniProt Secondary Accession:Q16695,Q6FGU4, B2R5K3,
UniProt Related Accession:Q16695
Molecular Weight:
NCBI Full Name:Histone H3.1t
NCBI Synonym Full Names:histone cluster 3, H3
NCBI Official Symbol:HIST3H3 
NCBI Official Synonym Symbols:H3t; H3.4; H3/g; H3FT 
NCBI Protein Information:histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name:Histone H3.1t
UniProt Synonym Protein Names:H3/g
UniProt Gene Name:HIST3H3 
UniProt Entry Name:H31T_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Other (Wide Range)
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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