Description
Acetyl-p53- K370 Monoclonal Antibody (CAB19836)
The Acetyl-p53- K370 Monoclonal Antibody (CAB19836) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody is raised in rabbits and is validated for use in various applications, including Western blotting and immunohistochemistry.p53 is a tumor suppressor protein that plays a crucial role in regulating cell growth and apoptosis. Acetylation of p53 at lysine 370 is known to affect its stability and activity, influencing cellular responses to stress and DNA damage.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | Acetyl-p53- K370 Monoclonal Antibody |
| SKU: | CAB19836 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Clone Number: | ARC2368 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | GGSR AHSS HLKS KKGQ STSR H | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | P53, BCC7, LFS1, BMFS5, TRP53, Acetyl-p53- K370 |
| Positive Sample: | HeLa treated with TSA, NIH/3T3 treated with TSA |
| Cellular Localization: | Cytoplasm, Endoplasmic Reticulum, Mitochondrion Matrix, Nucleus, Pml Body. |
| Calculated MW: | 44kDa |
| Observed MW: | 53kDa |
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
| Purification Method | Affinity purification |
| Gene ID | 7157 |
| RRID | AB_2862752 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using Acetyl-p53- K370 Rabbit mAb (CAB19836) at 1:1000 dilution. HeLa cells and NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 1s. |
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