Description
Phospho-p53-S392 Monoclonal Antibody (CABP1137)
The Phospho-p53-S392 Monoclonal Antibody (CABP1137) is a high-quality antibody developed for reliable detection and analysis of target proteins. P53 is a well-known tumor suppressor protein that plays a crucial role in cell cycle regulation and DNA repair. Phosphorylation of p53 at serine 392 is known to influence its activity and stability, making this antibody essential for studying the functional regulation of p53 in cancer and other disease contexts.This antibody, produced in rabbits, exhibits high specificity and sensitivity towards phospho-p53 (S392) in human samples, making it suitable for use in various applications such as Western blotting and immunohistochemistry.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
| Product Name: | Phospho-p53-S392 Monoclonal Antibody |
| SKU: | CABP1137 |
| Size: | 20μL, 100μL |
| Reactivity: | Rat |
| Clone Number: | ARC0309 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | PDSD | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | P53, BCC7, LFS1, BMFS5, TRP53, Phospho-p53-S392 |
| Positive Sample: | C6 treated with Paclitaxel |
| Cellular Localization: | Cytoplasm, Endoplasmic Reticulum, Mitochondrion Matrix, Nucleus, Pml Body. |
| Calculated MW: | 44kDa |
| Observed MW: | 53kDa |
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
| Purification Method | Affinity purification |
| Gene ID | 7157 |
| RRID | AB_2864005 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from C6 cells, using Phospho-p53-S392 (CABP1137) at 1:1000 dilution. C6 cells were treated with Paclitaxel (100 nM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
