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ACLY Rabbit Polyclonal Antibody (CAB15251)

SKU:
CAB15251
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:ACLY Rabbit Polyclonal Antibody
SKU:CAB15251
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_001087.2).
Sequence:FSSE VQFG HAGA CANQ ASET AVAK NQAL KEAG VFVP RSFD ELGE IIQS VYED LVAN GVIV PAQE VPPP TVPM DYSW AREL GLIR KPAS FMTS ICDE RGQE LIYA GMPI TEVF KEEM GIGG VLGL LWFQ KRLP KYSC QFIE MCLM VTAD HGPA VSGA HNTI ICAR AGKD LVSS LTSG LLTI GDRF GGAL DAAA KMFS KAFD SGII PMEF VNKM KKEG KLIM GIGH RVKS INNP DMRV QILK DYVR QHFP ATPL LDYA LEVE KITT SKKP NLIL NVDG LIGV AFVD MLRN CGSF TREE ADEY IDIG ALNG IFVL GRSM GFIG HYLD QKRL KQGL YRHP WDDI SYVL PEHM SM
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:ACL; ATPCL; CLATP; ACLY
Positive Sample:Jurkat,HeLa,Rat lung,Rat liver,Mouse lung,Mouse liver,Mouse brain
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:121kDa
Observed MW:125kDa

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Purification Method:Affinity purification
Gene ID:47
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ACLY Rabbit pAb (CAB15251) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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