Anti-ACSL3 Antibody (CAB11679)

Antibody Name:	Anti-ACSL3 Antibody
Antibody SKU:	CAB11679
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 42-300 of human ACSL3 (NP_004448.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 42-300 of human ACSL3 (NP_004448.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YFFS ESRQ EKSN RIKA KPVN SKPD SAYR SVNS LDGL ASVL YPGC DTLD KVFT YAKN KFKN KRLL GTRE VLNE EDEV QPNG KIFK KVIL GQYN WLSY EDVF VRAF NFGN GLQM LGQK PKTN IAIF CETR AEWM IAAQ ACFM YNFQ LVTL YATL GGPA IVHA LNET EVTN IITS KELL QTKL KDIV SLVP RLRH IITV DGKP PTWS EFPK GIIV HTMA AVEA LGAK ASME NQPH SKPL PSDI AVIM YTSG STGL PKGV MIS
Gene ID:	2181
Uniprot:	O95573
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein
Calculated MW:	80kDa
Observed MW:	75kDa

Synonyms:

ACSL3, ACS3, FACL3, PRO2194

Background:

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	ACSL3: Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). Belongs to the ATP-dependent AMP-binding enzyme family.
UniProt Protein Details:	Protein type:Ligase; Lipid Metabolism - fatty acid; Membrane protein, integral; EC 6.2.1.3 Chromosomal Location of Human Ortholog: 2q34-q35 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; lipid particle; membrane; perinuclear region of cytoplasm Molecular Function:long-chain-fatty-acid-CoA ligase activity; protein domain specific binding; protein kinase binding Biological Process: positive regulation of Golgi to plasma membrane protein transport; positive regulation of secretion
NCBI Summary:	The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O95573
NCBI GenInfo Identifier:	115502350
NCBI Gene ID:	2181
NCBI Accession:	O95573.3
UniProt Secondary Accession:	O95573,Q60I92, Q8IUM9,
UniProt Related Accession:	O95573
Molecular Weight:	80,420 Da
NCBI Full Name:	Long-chain-fatty-acid--CoA ligase 3
NCBI Synonym Full Names:	acyl-CoA synthetase long-chain family member 3
NCBI Official Symbol:	ACSL3
NCBI Official Synonym Symbols:	ACS3; FACL3; PRO2194
NCBI Protein Information:	long-chain-fatty-acid--CoA ligase 3
UniProt Protein Name:	Long-chain-fatty-acid--CoA ligase 3
UniProt Synonym Protein Names:	Long-chain acyl-CoA synthetase 3; LACS 3
Protein Family:	Long-chain-fatty-acid--CoA ligase
UniProt Gene Name:	ACSL3
UniProt Entry Name:	ACSL3_HUMAN

	Western blot analysis of extracts of various cell lines, using ACSL3 antibody (CAB11679) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using ACSL3 antibody (CAB11679) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using ACSL3 antibody (CAB11679) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using ACSL3 antibody (CAB11679) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using ACSL3 antibody (CAB11679) at dilution of 1:100 (40x lens).

Antibodies

ACSL3 Antibody (PACO07572)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)