Anti-ACSS2 Antibody (CAB6472)

Antibody Name:	Anti-ACSS2 Antibody
Antibody SKU:	CAB6472
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human ACSS2

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, COS-7, U-87MG, C2C12, Mouse liver, Mouse brain, Rat liver, Rat kidney

Immunogen:	Recombinant protein of human ACSS2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	55902
Uniprot:	Q9NR19
Cellular Location:	Cytoplasm
Calculated MW:	78kDa/80kDa
Observed MW:	78kDa

Synonyms:

ACSS2, ACAS2, ACECS, ACS, ACSA, dJ1161H23.1

Background:

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ACSS2: Activates acetate so that it can be used for lipid synthesis or for energy generation. Monomer. Belongs to the ATP-dependent AMP-binding enzyme family.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 6.2.1.1; Ligase Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm Molecular Function:acetate-CoA ligase activity; AMP binding Biological Process: ethanol oxidation; lipid biosynthetic process
NCBI Summary:	This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
UniProt Code:	Q9NR19
NCBI GenInfo Identifier:	20137525
NCBI Gene ID:	55902
NCBI Accession:	Q9NR19.1
UniProt Secondary Accession:	Q9NR19,Q5QPH2, Q5QPH3, Q8N238, Q96EL0, Q9NQP7, Q9UJ15 A6NE90,
UniProt Related Accession:	Q9NR19
Molecular Weight:	80,087 Da
NCBI Full Name:	Acetyl-coenzyme A synthetase, cytoplasmic
NCBI Synonym Full Names:	acyl-CoA synthetase short-chain family member 2
NCBI Official Symbol:	ACSS2
NCBI Official Synonym Symbols:	ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
NCBI Protein Information:	acetyl-coenzyme A synthetase, cytoplasmic
UniProt Protein Name:	Acetyl-coenzyme A synthetase, cytoplasmic
UniProt Synonym Protein Names:	Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme
Protein Family:	Acetyl-coenzyme A synthetase
UniProt Gene Name:	ACSS2
UniProt Entry Name:	ACSA_HUMAN

	Western blot analysis of extracts of various cell lines, using ACSS2 antibody (CAB6472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat heart using ACSS2 antibody (CAB6472) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using ACSS2 antibody (CAB6472) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of NIH-3T3 cells using ACSS2 Polyclonal Antibody (CAB6472) at dilution of 1:200. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using ACSS2 Rabbit pAb (CAB6472) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-ACSS2 Antibody (CAB13596)

Anti-ACSS2 Antibody (CAB12334)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)