α-Smooth Muscle Actin (ACTA2) Rabbit Polyclonal Antibody (CAB1011)
- SKU:
- CAB1011
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Product Name: | α-Smooth Muscle Actin (ACTA2) Rabbit Polyclonal Antibody |
| SKU: | CAB1011 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human α-Smooth Muscle Actin (ACTA2) (NP_001604.1). |
| Sequence: | MCEE EDST ALVC DNGS GLCK AGFA GDDA PRAV FPSI VGRP RHQG VMVG MGQK DSYV GDEA QSKR GILT LKYP IEHG IITN WDDM EKIW HHSF YNEL RVAP EEHP TLLT EAPL NPKA NREK MTQI |
| Tested Applications: | WB IHC-P IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:500 - 1:1000 IF/ICC,1:50 - 1:200 |
| Synonyms: | ACTSA; α-Smooth Muscle Actin (ACTA2) |
| Positive Sample: | Mouse heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, cytoskeleton. |
| Calculated MW: | 42kDa |
| Observed MW: | 42kDa |
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
| Purification Method: | Affinity purification |
| Gene ID: | 59 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Mouse heart, using α-Smooth Muscle Actin (ACTA2) Rabbit pAb (CAB1011) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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