Anti-ACTB Antibody (MACO0180)
- SKU:
- MACO0180
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Mouse
- NHP - Monkey
- Canine
- Host Species:
- Mouse
- Isotype:
- IgG1
- Applications:
- ELISA
- WB
- Antibody Type:
- Monoclonal Antibody
- Conjugation:
- Unconjugated
Description
| Product Name: | Anti-ACTB Antibody |
| Product Sku: | MACO0180 |
| Size: | 100ul |
| Host Species: | Mouse |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | WB:1:500-1:5000 |
| Species Reactivity: | Human, Rat, Mouse, Monkey, Dog |
| Immunogen: | Recombinant Protein |
| Form: | Liquid |
| Storage Buffer: | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Conjugate: | Non-conjugated |
| Background: | Beta-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against Beta-actin are useful as loading controls for Western Blotting. However it should be noted that levels of Beta-actin may not be stable in certain cells. For example, expression of Beta-actin in adipose tissue is very low and therefore Beta-actin should not be used as loading control for these tissues. |
| Synonyms: | ACTB; BRWS1; PS1TP5BP1 |
| UniProt Protein Function: | Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| UniProt Protein Details: | Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Ref.11 Ref.12 Ref.15 Ref.22 Subcellular location: Cytoplasm › cytoskeleton. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.17 Post-translational modification: ISGylated. Ref.13Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced By similarity. Involvement in Disease: Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [ MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. Ref.23Defects in ACTB are the cause of Baraitser-Winter syndrome type 1 (BRWS1) [ MIM:243310]. A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. Ref.25 Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Sequence similarities: Belongs to the actin family. |
| NCBI Summary: | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P60709 |
| NCBI GenInfo Identifier: | 46397333 |
| NCBI Gene ID: | 60 |
| NCBI Accession: | P60709.1 |
| UniProt Secondary Accession: | P60709,P02570, P70514, P99021, Q11211, Q64316, Q75MN2 Q96B34, Q96HG5, |
| UniProt Related Accession: | P60709 |
| Molecular Weight: | 67 KD |
| NCBI Full Name: | Actin, cytoplasmic 1 |
| NCBI Synonym Full Names: | actin, beta |
| NCBI Official Symbol: | ACTB |
| NCBI Official Synonym Symbols: | BRWS1; PS1TP5BP1 |
| NCBI Protein Information: | actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1 |
| UniProt Protein Name: | Actin, cytoplasmic 1 |
| UniProt Synonym Protein Names: | Beta-actin |
| Protein Family: | Actin |
| UniProt Gene Name: | ACTB |
| UniProt Entry Name: | ACTB_HUMAN |
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