Anti-ADAM11 Antibody (CAB14249)

Antibody Name:	Anti-ADAM11 Antibody
Antibody SKU:	CAB14249
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 290-430 of human ADAM11 (NP_002381.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 290-430 of human ADAM11 (NP_002381.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METW ADGD KIQV QDDL LETL ARLM VYRR EGLP EPSD ATHL FSGR TFQS TSSG AAYV GGIC SLSH GGGV NEYG NMGA MAVT LAQT LGQN LGMM WNKH RSSA GDCK CPDI WLGC IMED TGFY LPRK FSRC SIDE YNQF LQEG G
Gene ID:	4185
Uniprot:	O75078
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	57kDa/83kDa
Observed MW:	83kDa

Synonyms:

ADAM11, MDC

Background:

This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. This gene represents a candidate tumor suppressor gene for human breast cancer based on its location within a minimal region of chromosome 17q21 previously defined by tumor deletion mapping. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

UniProt Protein Function:	ADAM11: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Protease; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q21.3 Cellular Component: extracellular matrix; plasma membrane Molecular Function:integrin binding; metallopeptidase activity Biological Process: integrin-mediated signaling pathway
NCBI Summary:	This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. This gene represents a candidate tumor suppressor gene for human breast cancer based on its location within a minimal region of chromosome 17q21 previously defined by tumor deletion mapping. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Code:	O75078
NCBI GenInfo Identifier:	115502351
NCBI Gene ID:	4185
NCBI Accession:	O75078.3
UniProt Secondary Accession:	O75078,Q14808, Q14809, Q14810,
UniProt Related Accession:	O75078,AAB29191
Molecular Weight:	57,911 Da
NCBI Full Name:	Disintegrin and metalloproteinase domain-containing protein 11
NCBI Synonym Full Names:	ADAM metallopeptidase domain 11
NCBI Official Symbol:	ADAM11
NCBI Official Synonym Symbols:	MDC
NCBI Protein Information:	disintegrin and metalloproteinase domain-containing protein 11
UniProt Protein Name:	Disintegrin and metalloproteinase domain-containing protein 11
UniProt Synonym Protein Names:	Metalloproteinase-like, disintegrin-like, and cysteine-rich protein; MDC
Protein Family:	Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name:	ADAM11
UniProt Entry Name:	ADA11_HUMAN

	Western blot analysis of extracts of various cell lines, using ADAM11 antibody (CAB14249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of C6 cells using ADAM11 antibody (CAB14249) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using ADAM11 antibody (CAB14249) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)