The ADAM9 Antibody (CAB5388) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has been specifically designed for use in Western blot applications and is highly reactive with human samples.ADAM9 is known to play a role in various cellular processes, including cell adhesion, migration, and signaling. It is also implicated in the pathogenesis of cancer, with increased expression levels observed in various types of cancer, making it a potential target for cancer therapy.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.
Product Name:
ADAM9 Antibody
SKU:
CAB5388
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Monkey
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCMP, MDC9, CORD9, Mltng, ADAM9
Positive Sample:
BxPC-3, Mouse liver, Mouse brain, COS-7
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
91kDa
Observed MW:
100kDa
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene.
Purification Method
Affinity purification
Gene ID
8754
RRID
AB_2766197
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ADAM9 Rabbit pAb (CAB5388) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from COS-7 cells using ADAM9 Rabbit pAb (CAB5388) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of PC-12 cells using ADAM9 Rabbit pAb (CAB5388) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
