Anti-AEBP1 Antibody (CAB16340)

Antibody Name:	Anti-AEBP1 Antibody
Antibody SKU:	CAB16340
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 999-1158 of human AEBP1 (NP_001120.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 999-1158 of human AEBP1 (NP_001120.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NRPI PHID PSRP MTPQ QRRL QQRR LQHR LRLR AQMR LRRL NATT TLGP HTVP PTLP PAPA TTLS TTIE PWGL IPPT TAGW EESE TETY TEVV TEFG TEVE PEFG TKVE PEFE TQLE PEFE TQLE PEFE EEEE EEKE EEIA TGQA FPFT TVET YTVN FGDF
Gene ID:	165
Uniprot:	Q8IUX7
Cellular Location:	Cytoplasm, Nucleus, Secreted
Calculated MW:	82kDa/130kDa
Observed MW:	131kDa

Synonyms:	AEBP1, ACLP
Background:	This gene encodes a member of carboxypeptidase A protein family. The encoded protein may function as a transcriptional repressor and play a role in adipogenesis and smooth muscle cell differentiation. Studies in mice suggest that this gene functions in wound healing and abdominal wall development. Overexpression of this gene is associated with glioblastoma.

UniProt Protein Function:	AEBP1: May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I- kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 7p13 Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleus Molecular Function:calmodulin binding; carboxypeptidase activity; zinc ion binding; transcription factor activity; metallocarboxypeptidase activity; transcription corepressor activity Biological Process: muscle development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; proteolysis; skeletal development
NCBI Summary:	This gene encodes a member of carboxypeptidase A protein family. The encoded protein may function as a transcriptional repressor and play a role in adipogenesis and smooth muscle cell differentiation. Studies in mice suggest that this gene functions in wound healing and abdominal wall development. Overexpression of this gene is associated with glioblastoma. [provided by RefSeq, May 2013]
UniProt Code:	Q8IUX7
NCBI GenInfo Identifier:	119581523
NCBI Gene ID:	165
NCBI Accession:	EAW61119.1
UniProt Secondary Accession:	Q8IUX7,Q14113, Q59ER7, Q6ZSC7, Q7KZ79,
UniProt Related Accession:	Q8IUX7
Molecular Weight:	82,183 Da
NCBI Full Name:	AE binding protein 1
NCBI Synonym Full Names:	AE binding protein 1
NCBI Official Symbol:	AEBP1
NCBI Official Synonym Symbols:	ACLP
NCBI Protein Information:	adipocyte enhancer-binding protein 1
UniProt Protein Name:	Adipocyte enhancer-binding protein 1
UniProt Synonym Protein Names:	Aortic carboxypeptidase-like protein
Protein Family:	Adipocyte enhancer-binding protein
UniProt Gene Name:	AEBP1
UniProt Entry Name:	AEBP1_HUMAN

	Western blot analysis of extracts of mouse brain, using AEBP1 antibody (CAB16340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using AEBP1 Polyclonal Antibody (CAB16340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using AEBP1 Polyclonal Antibody (CAB16340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using AEBP1 Polyclonal Antibody (CAB16340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ELISA Kits

Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)