AFG1L Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
AFG1L Antibody (PACO38066)
SKU:
PACO38066
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human, Rat
Immunogen:
Recombinant Human AFG1-like ATPase protein (14-313AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
Q8WV93
Form:
Liquid
Tested Applications:
ELISAWBIHCIF
Recommended Dilution:
WB 1:500-1:5000, IHC 1:500-1:1000, IF 1:200-1:500
Synonyms:
AFG1 antibody, ATPase family gene 1 homolog antibody, CG8520 gene product antibody, lace1 antibody, LACE1_HUMAN antibody, Lactation elevated protein 1 antibody, Lactivation elevated 1 antibody, Protein AFG1 homolog antibody
Western Blot Positive WB detected in: A549 whole cell lysate, Rat kidney tissue All lanes: AFG1L antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
IHC image of PACO38066 diluted at 1:600 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO38066 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO38066 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
