Anti-AGFG1 Antibody (CAB13500)

Antibody Name:	Anti-AGFG1 Antibody
Antibody SKU:	CAB13500
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAS AKRK QEEK HLKM LRDM TGLP HNRK CFDC DQRG PTYV NMTV GSFV CTSC SGSL RGLN PPHR VKSI SMTT FTQQ EIEF LQKH GNEV CKQI WLGL FDDR SSAI PDFR DPQK VKEF LQEK YEKK RWYV PPEQ AKVV ASVH ASIS GSSA SSTS STPE VKPL KSLL GDSA PTLH LNKG TPSQ SPVV GRSQ GQQQ EKKQ FDLL SDLG SDIF AAPA PQST ATAN FANF AHFN SHAA QNSA NADF ANFD AFGQ SSGS SNFG GFPT ASHS PFQP QTTG GSAA SVNA
Gene ID:	3267
Uniprot:	P52594
Cellular Location:	Cytoplasmic vesicle, Nucleus
Calculated MW:	54kDa/58kDa/60kDa
Observed MW:	58kDa

Synonyms:

AGFG1, HRB, RAB, RIP

Background:

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RIP: Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. Interacts with EPS15R and EPS15. Interacts with FCHO1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear export; RNA-binding Chromosomal Location of Human Ortholog: 2q36.3 Cellular Component: intracellular membrane-bound organelle; nuclear pore Molecular Function:protein binding; RNA binding Biological Process: mRNA export from nucleus
NCBI Summary:	The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Code:	P52594
NCBI GenInfo Identifier:	26007019
NCBI Gene ID:	3267
NCBI Accession:	P52594.2
UniProt Secondary Accession:	P52594,Q15277, Q4VAS0, Q4VAS1, Q4VAS3, Q53QT8, Q53R11 B3KUL1, E9PHX7,
UniProt Related Accession:	P52594
Molecular Weight:	60,767 Da
NCBI Full Name:	Arf-GAP domain and FG repeat-containing protein 1
NCBI Synonym Full Names:	ArfGAP with FG repeats 1
NCBI Official Symbol:	AGFG1
NCBI Official Synonym Symbols:	HRB; RAB; RIP
NCBI Protein Information:	arf-GAP domain and FG repeat-containing protein 1
UniProt Protein Name:	Arf-GAP domain and FG repeat-containing protein 1
UniProt Synonym Protein Names:	HIV-1 Rev-binding protein; Nucleoporin-like protein RIP; Rev-interacting protein; Rev/Rex activation domain-binding protein
Protein Family:	Riparin
UniProt Gene Name:	AGFG1

	Western blot analysis of extracts of various cell lines, using AGFG1 antibody (CAB13500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using AGFG1 antibody (CAB13500). Blue: DAPI for nuclear staining.

Antibodies

Anti-AGFG1 Antibody (CAB6294)

AGFG1 Antibody (PACO07640)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)