The Argonaute-2 Monoclonal Antibody (CAB19709) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot and immunohistochemistry applications.Argonaute 2 is a crucial component of the RNA-induced silencing complex (RISC), playing a central role in gene regulation and silencing through microRNA-mediated mechanisms. Its involvement in post-transcriptional gene silencing makes it a promising target for investigating various cellular processes, including development, differentiation, and disease pathways.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Argonaute-2 Monoclonal Antibody
SKU:
CAB19709
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0189
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PPD, Q10, CASC7, EIF2C2, LESKRES, LINC00980, Ago 2, Argonaute-2
Positive Sample:
293T, U-87MG, MCF7, HepG2, Mouse kidney, Rat liver
Cellular Localization:
Cytoplasm, Nucleus, P-Body.
Calculated MW:
97kDa
Observed MW:
97kDa
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
27161
RRID
AB_2862747
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Argonaute-2 Rabbit mAb (CAB19709) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Argonaute-2 Rabbit mAb (CAB19709) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Argonaute-2 Rabbit mAb (CAB19709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Argonaute-2 Rabbit mAb (CAB19709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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