The Argonaute-2 Monoclonal Antibody (CAB19709) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2862747 Gene ID 27161 Swiss Prot Synonym PPD; Q10; CASC7; EIF2C2; LESKRES; LINC00980; Ago 2; Argonaute-2
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Argonaute-2 Monoclonal Antibody
SKU:
CAB19709
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0189
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:100 - 1:400
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PPD, Q10, CASC7, EIF2C2, LESKRES, LINC00980, Ago 2, Argonaute-2
Positive Sample:
293T, U-87MG, MCF7, HepG2, Mouse kidney, Rat liver
Cellular Localization:
Cytoplasm, Nucleus, P-Body.
Calculated MW:
97kDa
Observed MW:
97kDa
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2862747 Gene ID 27161 Swiss Prot Synonym PPD; Q10; CASC7; EIF2C2; LESKRES; LINC00980; Ago 2; Argonaute-2
Purification Method:
Affinity purification
Gene ID:
27161
RRID:
AB_2862747
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Argonaute-2 Rabbit mAb (CAB19709) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Argonaute-2 Rabbit mAb (CAB19709) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Argonaute-2 Rabbit mAb (CAB19709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Argonaute-2 Rabbit mAb (CAB19709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
