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Anti-AHR Antibody (CAB1451)

SKU:
CAB1451
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 5 Anti-AHR Antibody CAB1451
  • Cell Biology Antibodies 5 Anti-AHR Antibody CAB1451
€139 - €419
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Description

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Antibody Name:Anti-AHR Antibody
Antibody SKU:CAB1451
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 619-848 of human AHR (NP_001612.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HepG2, 293T, SW480, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 619-848 of human AHR (NP_001612.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LEQQ QQHH QKQV VVEP QQQL CQKM KHMQ VNGM FENW NSNQ FVPF NCPQ QDPQ QYNV FTDL HGIS QEFP YKSE MDSM PYTQ NFIS CNQP VLPQ HSKC TELD YPMG SFEP SPYP TTSS LEDF VTCL QLPE NQKH GLNP QSAI ITPQ TCYA GAVS MYQC QPEP QHTH VGQM QYNP VLPG QQAF LNKF QNGV LNET YPAE LNNI NNTQ TTTH LQPL HHPS EARP FPDL TSSG FL
Gene ID:196
Uniprot:P35869
Cellular Location:Cytoplasm, Nucleus
Calculated MW:96kDa
Observed MW:115kDa
Synonyms:AHR, bHLHe76
Background:The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.
UniProt Protein Function:AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes. AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function:protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; Hsp90 protein binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; prostate gland development; blood vessel development; intracellular receptor-mediated signaling pathway; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; cell cycle; regulation of transcription from RNA polymerase II promoter; response to xenobiotic stimulus; regulation of transcription, DNA-dependent; regulation of gene expression; xenobiotic metabolic process; regulation of B cell proliferation; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly

NCBI Summary:This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq, Jul 2008]
UniProt Code:P35869
NCBI GenInfo Identifier:3041653
NCBI Gene ID:196
NCBI Accession:P35869.2
UniProt Secondary Accession:P35869,Q13728, Q13803, Q13804, A4D130,
UniProt Related Accession:P35869
Molecular Weight:848
NCBI Full Name:Aryl hydrocarbon receptor
NCBI Synonym Full Names:aryl hydrocarbon receptor
NCBI Official Symbol:AHR  
NCBI Official Synonym Symbols:bHLHe76  
NCBI Protein Information:aryl hydrocarbon receptor; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76
UniProt Protein Name:Aryl hydrocarbon receptor
UniProt Synonym Protein Names:Class E basic helix-loop-helix protein 76; bHLHe76
Protein Family:Aldehyde reductase
UniProt Gene Name:AHR  
UniProt Entry Name:AHR_HUMAN
Anti-AHR Antibody (CAB1451)Western blot analysis of extracts of various cell lines, using AHR antibody (CAB1451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-AHR Antibody (CAB1451)Immunohistochemistry of paraffin-embedded human stomach using AHR antibody (CAB1451) at dilution of 1:100 (40x lens).

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