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Anti-PODXL Antibody (CAB4554)

SKU:
CAB4554
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
Gp200
PC
PCLP
PCLP-1
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

Product Name:PODXL Rabbit mAb
Product Code:CAB4554
Size:20uL, 50uL, 100uL
Synonyms:Gp200, PC, PCLP, PCLP-1
Applications:WB, IHC
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human PODXL
Applications:WB, IHC
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human
Positive Samples:HCT116, 293T, U-251MG
Immunogen:A synthesized peptide derived from human PODXL
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5420
Uniprot:O00592
Calculated MW:165kDa
Observed MW:200-400KDa
UniProt Protein Function:PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 7q32-q33

Cellular Component: ruffle; extracellular space; microvillus membrane; lamellipodium; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; lipid raft; filopodium

Molecular Function:protein binding

Biological Process: cell migration; negative regulation of cell-cell adhesion; negative regulation of cell adhesion; cell adhesion; positive regulation of cell-cell adhesion mediated by integrin; leukocyte migration; regulation of microvillus biogenesis; positive regulation of cell migration

NCBI Summary:This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]
UniProt Code:O00592
NCBI GenInfo Identifier:229462740
NCBI Gene ID:5420
NCBI Accession:O00592.2
UniProt Secondary Accession:O00592,Q52LZ7, Q53ER6, A6NHX8,
UniProt Related Accession:O00592
Molecular Weight:558
NCBI Full Name:Podocalyxin
NCBI Synonym Full Names:podocalyxin-like
NCBI Official Symbol:PODXL  
NCBI Official Synonym Symbols:PC; PCLP; Gp200; PCLP-1  
NCBI Protein Information:podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1
UniProt Protein Name:Podocalyxin
UniProt Synonym Protein Names:GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1
Protein Family:Podocalyxin
UniProt Gene Name:PODXL  
UniProt Entry Name:PODXL_HUMAN

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