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Anti-AK2 Antibody [KO Validated] (CAB20934)

SKU:
CAB20934
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
  • Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from normal (control) and AK2 knockout (KO) HeLa cells, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
€179 - €469
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Description

Antibody Name:Anti-AK2 Antibody [KO Validated] (CAB20934)
Antibody SKU:CAB20934
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human AK2.
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat kidney
Immunogen:Recombinant protein of Human AK2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Mitochondrion intermembrane space
Calculated MW:14kDa/21kDa/22kDa/24kDa/25kDa/26kDa
Observed MW:26KDa
Synonyms:AK2, ADK2
Background:Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF7 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of MCF7 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HepG2 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts from normal (control) and AK2 knockout (KO) HeLa cells, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Western blot analysis of extracts from normal (control) and AK2 knockout (KO) HeLa cells, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Western blot analysis of extracts of various cell lines, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

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