Anti-ALDH6A1 Antibody (CAB3309)

Antibody Name:	Anti-ALDH6A1 Antibody
Antibody SKU:	CAB3309
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 326-535 of human ALDH6A1 (NP_005580.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, A-431, SH-SY5Y, 22Rv1, MCF7, Mouse liver, Mouse kidney, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 326-535 of human ALDH6A1 (NP_005580.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VGEA KKWL PELV EHAK NLRV NAGD QPGA DLGP LITP QAKE RVCN LIDS GTKE GASI LLDG RKIK VKGY ENGN FVGP TIIS NVKP NMTC YKEE IFGP VLVV LETE TLDE AIQI VNNN PYGN GTAI FTTN GATA RKYA HLVD VGQV GVNV PIPV PLPM FSFT GSRS SFRG DTNF YGKQ GIQF YTQL KTIT SQWK EEDA TLSS PAVV MPTM GR
Gene ID:	4329
Uniprot:	Q02252
Cellular Location:	Mitochondrion
Calculated MW:	56kDa/57kDa
Observed MW:	58kDa

Synonyms:

ALDH6A1, MMSADHA, MMSDH

Background:

This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	ALDH6A1: Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA. Defects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDHD). This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Belongs to the aldehyde dehydrogenase family.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - inositol phosphate; EC 1.2.1.27; EC 1.2.1.18; Mitochondrial; Oxidoreductase; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix Molecular Function:aldehyde dehydrogenase (NAD) activity; acyl-CoA binding; methylmalonate-semialdehyde dehydrogenase (acylating) activity; malonate-semialdehyde dehydrogenase (acetylating) activity Biological Process: valine catabolic process; thymine metabolic process; brown fat cell differentiation; thymine catabolic process; valine metabolic process; branched chain family amino acid catabolic process Disease: Methylmalonate Semialdehyde Dehydrogenase Deficiency
NCBI Summary:	This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
UniProt Code:	Q02252
NCBI GenInfo Identifier:	12643424
NCBI Gene ID:	4329
NCBI Accession:	Q02252.2
UniProt Secondary Accession:	Q02252,Q9UKM8, B2R609, B4DFS8, J3KNU8,
UniProt Related Accession:	Q02252
Molecular Weight:	535
NCBI Full Name:	Methylmalonate-semialdehyde dehydrogenase
NCBI Synonym Full Names:	aldehyde dehydrogenase 6 family, member A1
NCBI Official Symbol:	ALDH6A1
NCBI Official Synonym Symbols:	MMSDH; MMSADHA
NCBI Protein Information:	methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; mitochondrial acylating methylmalonate-semialdehyde dehydrogenase
UniProt Protein Name:	Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
UniProt Synonym Protein Names:	Aldehyde dehydrogenase family 6 member A1
UniProt Gene Name:	ALDH6A1
UniProt Entry Name:	MMSA_HUMAN

	Western blot analysis of extracts of various cell lines, using ALDH6A1 antibody (CAB3309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using ALDH6A1 antibody (CAB3309) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using ALDH6A1 antibody (CAB3309) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-ALDH6A1 Antibody (CAB14750)

ALDH6A1 Antibody (PACO07689)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)