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Anti-ALG1 Antibody (CAB7818)

SKU:
CAB7818
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 11 Anti-ALG1 Antibody CAB7818
  • Cell Biology Antibodies 11 Anti-ALG1 Antibody CAB7818
€139 - €419
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Description

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Antibody Name:Anti-ALG1 Antibody
Antibody SKU:CAB7818
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, Mouse liver, Mouse kidney, Mouse ovary, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YDKP ASFF KETP LDLQ HRLF MKLG SMHS PFRA RSEP EDPV TERS AFTE RDAG SGLV TRLR ERPA LLVS STSW TEDE DFSI LLAA LEKF EQLT LDGH NLPS LVCV ITGK GPLR EYYS RLIH QKHF QHIQ VCTP WLEA EDYP LLLG SADL GVCL HTSS SGLD LPMK VVDM FGCC LPVC AVNF KCLH ELVK HEEN GLVF EDSE ELAA QLQM LFSN FPDP AGKL NQFR KNLR ESQQ LRWD ESWV QTVL PLVM DT
Gene ID:56052
Uniprot:Q9BT22
Cellular Location:Endoplasmic reticulum membrane, Single-pass type II membrane protein
Calculated MW:40kDa/52kDa
Observed MW:60kDa
Synonyms:ALG1, CDG1K, HMAT1, HMT-1, HMT1, MT-1, Mat-1, hMat-1
Background:The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.
UniProt Protein Function:ALG1: Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.142; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function:mannosyltransferase activity; chitobiosyldiphosphodolichol beta-mannosyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; lipopolysaccharide biosynthetic process; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Congenital Disorder Of Glycosylation, Type Ik

NCBI Summary:The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik. [provided by RefSeq, Dec 2008]
UniProt Code:Q9BT22
NCBI GenInfo Identifier:73921663
NCBI Gene ID:56052
NCBI Accession:Q9BT22.2
UniProt Related Accession:Q9BT22
Molecular Weight:Observed: 53 kDaPredicted: 53 kDa
NCBI Full Name:Chitobiosyldiphosphodolichol beta-mannosyltransferase
NCBI Synonym Full Names:ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase
NCBI Official Symbol:ALG1  
NCBI Official Synonym Symbols:HMT1; MT-1; CDG1K; HMAT1; HMT-1; Mat-1; hMat-1  
NCBI Protein Information:chitobiosyldiphosphodolichol beta-mannosyltransferase
UniProt Protein Name:Chitobiosyldiphosphodolichol beta-mannosyltransferase
UniProt Synonym Protein Names:Asparagine-linked glycosylation protein 1 homolog; Beta-1,4-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransferase; Mannosyltransferase-1; MT-1; hMat-1
UniProt Gene Name:ALG1  
UniProt Entry Name:ALG1_HUMAN
Anti-ALG1 Antibody (CAB7818)Western blot analysis of extracts of various cell lines, using ALG1 antibody (CAB7818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ALG1 Antibody (CAB7818)Immunofluorescence analysis of A549 cells using ALG1 antibody (CAB7818). Blue: DAPI for nuclear staining.

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