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[KO Validated] ENO1 Rabbit Polyclonal Antibody (CAB1033)

SKU:
CAB1033
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • KO Validated Antibodies 1 Anti-ENO1 Antibody CAB1033KO Validated
  • KO Validated Antibodies 1 Anti-ENO1 Antibody CAB1033KO Validated
  • KO Validated Antibodies 1 Anti-ENO1 Antibody CAB1033KO Validated
  • KO Validated Antibodies 1 Anti-ENO1 Antibody CAB1033KO Validated
  • KO Validated Antibodies 1 Anti-ENO1 Antibody CAB1033KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] ENO1 Rabbit Polyclonal Antibody
SKU:CAB1033
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1).
Sequence:MSIL KIHA REIF DSRG NPTV EVDL FTSK GLFR AAVP SGAS TGIY EALE LRDN DKTR YMGK GVSK AVEH INKT IAPA LVSK KLNV TEQE KIDK LMIE MDGT ENKS KFGA NAIL GVSL AVCK AGAV EKGV PLYR HIAD LAGN SEVI LPVP AFNV INGG SHAG NKLA MQEF MILP VGAA NFRE AMRI GAEV YHNL KNVI KEKY GKDA TNVG DEGG FAPN ILEN KEGL ELLK TAIG KAGY TDKV VIGM DVAA SEFF RSGK YDLD FKSP DDPS RYIS PDQL ADLY KSFI KDYP VVSI EDPF DQDD WGAW QKFT ASAG IQVV GDDL TVTN PKRI AKAV NEKS CNCL LLKV NQIG SVTE SLQA CKLA QANG WGVM VSHR SGET EDTF IADL VVGL CTGQ IKTG APCR SERL AKYN QLLR IEEE LGSK AKFA GRNF RNPL AK
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:NNE; PPH; MPB1; ENO1L1; ENO1-IT1; HEL-S-17;ENO1
Positive Sample:HeLa,SH-SY5Y,MCF-7,Jurkat,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, M line, Nucleus, myofibril, sarcomere.
Calculated MW:47kDa
Observed MW:47kDa/

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Purification Method:Affinity purification
Gene ID:2023
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ENO1 Rabbit pAb (CAB1033) at 1:3000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 1 s.

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