Anti-ALPP Antibody (CAB6353)

Antibody Name:	Anti-ALPP Antibody
Antibody SKU:	CAB6353
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human ALPP (NP_001623.3).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human ALPP (NP_001623.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LAKR QGAR YVWN RTEL MQAS LDPS VTHL MGLF EPGD MKYE IHRD STLD PSLM EMTE AALR LLSR NPRG FFLF VEGG RIDH GHHE SRAY RALT ETIM FDDA IERA GQLT SEED TLSL VTAD HSHV FSFG GYPL RGSS IFGL APGK ARDR KAYT VLLY GNGP GYVL KDGA RPDV TESE SGSP EYRQ QSAV PLDE ETHA GEDV A
Gene ID:	250
Uniprot:	P05187
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:	57kDa
Observed MW:	70kDa

Synonyms:

ALPP, ALP, PALP, PLAP, PLAP-1, placental

Background:

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

UniProt Protein Function:	ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Cell surface; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor; EC 3.1.3.1 Chromosomal Location of Human Ortholog: 2q37 Cellular Component: cell surface; plasma membrane; integral to membrane Molecular Function:zinc ion binding; alkaline phosphatase activity; magnesium ion binding Biological Process: dephosphorylation
NCBI Summary:	There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P05187
NCBI GenInfo Identifier:	94721246
NCBI Gene ID:	250
NCBI Accession:	NP_001623.3
UniProt Secondary Accession:	P05187,P05188, P06861, Q53S78, Q96DB7,
UniProt Related Accession:	P05187
Molecular Weight:	57,954 Da
NCBI Full Name:	alkaline phosphatase, placental type preproprotein
NCBI Synonym Full Names:	alkaline phosphatase, placental
NCBI Official Symbol:	ALPP
NCBI Official Synonym Symbols:	ALP; PALP; PLAP; PLAP-1
NCBI Protein Information:	alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme
UniProt Protein Name:	Alkaline phosphatase, placental type
UniProt Synonym Protein Names:	Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1
Protein Family:	Phosphatase
UniProt Gene Name:	ALPP
UniProt Entry Name:	PPB1_HUMAN

	Western blot analysis of extracts of HeLa cells, using ALPP antibody (CAB6353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human placenta using ALPP antibody (CAB6353) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug ALPP antibody (CAB6353). Western blot was performed from the immunoprecipitate using ALPP antibody (CAB6353) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-Placental alkaline phosphatase (PLAP) Antibody (CAB4304)	Human PLAP / Placental alkaline phosphatase ELISA Kit
ALPP Antibody (PACO07708)	Human PLAP/ALPP (Placental Alkaline Phosphatase) CLIA Kit (HUES01075)
	Human Alkaline phosphatase, placental type (ALPP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)