Description
| Product Name: | ALT/GPT Activity Assay Kit - Fluorometric |
| Product Code: | MAES0009 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 75 min |
| Instrument: | Fluorescence Microplate Reader |
| Sensitivity: | 0.01 U/L |
| Detection Range: | 0.01-0.83 U/L |
| Inter CV: | 9.8 |
| Inter CV: | 2.3 |
Alanine aminotransferase (ALT) is widely found in plasma and various tissues of the body, including liver, kidney, heart and skeletal muscle. ALT is an important pyridoxal phosphate dependent enzyme in the intermediate metabolism of glucose and protein. Clinically, the activity of serum alanine aminotransferase is often used as a marker for alcoholic liver disease, liver cirrhosis and acute viral hepatitis.
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Liver Biomarkers |
