Anti-Ambra1 Antibody (CAB12578)

Antibody Name:	Anti-Ambra1 Antibody
Antibody SKU:	CAB12578
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 961-1208 of human Ambra1 (NP_060219.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 961-1208 of human Ambra1 (NP_060219.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QLNE TVFT VHSN SRSS ERPG TSRA TWRT DRDM GLMN AIGL QPRN PATS VTSQ GTQT LALQ LQNA ETQT EREV PEPG TAAS GPGE GEGS EYGA SGED ALSR IQRL MAEG GMTA VVQR EQST TMAS MGGF GNNI IVSH RIHR SSQT GTEP GAAH TSSP QPST SRGL LPEA GQLA ERGL SPRT ASWD QPGT PGRE PTQP TLPS SSPV PIPV SLPS AEGP TLHC ELTN NNHL LDGG SSRG DAAG PRGE PRNR
Gene ID:	55626
Uniprot:	Q9C0C7
Cellular Location:	Cytoplasmic vesicle, autophagosome
Calculated MW:	84kDa/132kDa/135kDa/139kDa/142kDa
Observed MW:	175kDa

Synonyms:	AMBRA1, DCAF3, WDR94, FLJ20294
Background:

UniProt Protein Function:	AMBRA1: a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Autophagy; Vesicle Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; autophagic vacuole; phagocytic vesicle; axoneme; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: neural tube development; negative regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; autophagy; negative regulation of neuron apoptosis; cell differentiation
UniProt Code:	Q9C0C7
NCBI GenInfo Identifier:	166215833
NCBI Gene ID:	55626
NCBI Accession:	Q9C0C7.2
UniProt Secondary Accession:	Q9C0C7,A2AH22,
UniProt Related Accession:	Q9C0C7
Molecular Weight:
NCBI Full Name:	Activating molecule in BECN1-regulated autophagy protein 1
NCBI Synonym Full Names:	autophagy and beclin 1 regulator 1
NCBI Official Symbol:	AMBRA1
NCBI Official Synonym Symbols:	DCAF3; WDR94
NCBI Protein Information:	activating molecule in BECN1-regulated autophagy protein 1
UniProt Protein Name:	Activating molecule in BECN1-regulated autophagy protein 1
Protein Family:	Activating molecule in BECN1-regulated autophagy protein
UniProt Gene Name:	AMBRA1
UniProt Entry Name:	AMRA1_HUMAN

Western blot analysis of extracts of mouse brain, using Ambra1 Antibody (CAB12578) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Antibodies

Anti-Ambra1 Antibody (CAB1083)

AMBRA1 Antibody (PACO07710)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)