The Ambra1 Antibody (CAB12578) is a high-quality antibody developed for reliable detection and analysis of target proteins. Ambra1, a key regulator of autophagy and cell survival, is essential for maintaining cellular homeostasis and responding to stressors. This polyclonal antibody, generated in rabbits, is highly specific for Ambra1 and has been validated for use in Western blot and immunofluorescence applications.Ambra1 is known for its involvement in the autophagic process, a cellular mechanism that clears damaged organelles and proteins to maintain cell health. Dysregulation of autophagy has been linked to various diseases, including neurodegenerative disorders and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Ambra1 Antibody
SKU:
CAB12578
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DCAF3, WDR94, Ambra1
Positive Sample:
Mouse brain
Cellular Localization:
Cytoplasmic Vesicle, Autophagosome.
Calculated MW:
143kDa
Observed MW:
175kDa
Enables GTPase binding activity and ubiquitin protein ligase binding activity. Involved in macroautophagy; positive regulation of phosphatidylinositol 3-kinase activity; and response to mitochondrial depolarisation. Located in cytosol. Colocalizes with mitochondrion. Biomarker of multiple system atrophy.
Purification Method
Affinity purification
Gene ID
55626
RRID
AB_2759420
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse brain, using Ambra1 Rabbit pAb (CAB12578) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
