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Anti-GFPT2 Antibody (CAB15374)

SKU:
CAB15374
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 6 Anti-GFPT2 Antibody CAB15374
  • Cell Biology Antibodies 6 Anti-GFPT2 Antibody CAB15374
€139 - €419
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Description

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Antibody Name:Anti-GFPT2 Antibody
Antibody SKU:CAB15374
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-260 of human GFPT2 (NP_005101.1).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, A375, NIH/3T3, Mouse heart, Mouse pancreas, Mouse testis, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-260 of human GFPT2 (NP_005101.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RGSP LLIG VRSK YKLS TEQI PILY RTCT LENV KNIC KTRM KRLD SSAC LHAV GDKA VEFF F
Gene ID:9945
Uniprot:O94808
Cellular Location:
Calculated MW:76kDa
Observed MW:77kDa
Synonyms:GFPT2, GFAT, GFAT 2, GFAT2
Background:
UniProt Protein Function:GFAT2: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Highest levels of expression in heart, placenta, and spinal cord.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.16; Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 5q34-q35

Cellular Component: cytosol

Molecular Function:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; protein binding

Biological Process: energy reserve metabolic process; fructose 6-phosphate metabolic process; UDP-N-acetylglucosamine biosynthetic process; UDP-N-acetylglucosamine metabolic process

UniProt Code:O94808
NCBI GenInfo Identifier:6226820
NCBI Gene ID:9945
NCBI Accession:O94808.3
UniProt Secondary Accession:O94808,Q53XM2, Q9BWS4,
UniProt Related Accession:O94808
Molecular Weight:76,931 Da
NCBI Full Name:Glutamine--fructose-6-phosphate aminotransferase
NCBI Synonym Full Names:glutamine-fructose-6-phosphate transaminase 2
NCBI Official Symbol:GFPT2  
NCBI Official Synonym Symbols:GFAT; GFAT2; GFAT 2  
NCBI Protein Information:glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
UniProt Protein Name:Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
UniProt Synonym Protein Names:D-fructose-6-phosphate amidotransferase 2; Glutamine:fructose-6-phosphate amidotransferase 2; GFAT 2; GFAT2; Hexosephosphate aminotransferase 2
Protein Family:Glutamine--fructose-6-phosphate aminotransferase
UniProt Gene Name:GFPT2  
UniProt Entry Name:GFPT2_HUMAN
Anti-GFPT2 Antibody (CAB15374)Western blot analysis of extracts of various cell lines, using GFPT2 antibody (CAB15374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-GFPT2 Antibody (CAB15374)Immunofluorescence analysis of L929 cells using GFPT2 antibody (CAB15374) at dilution of 1:100. Blue: DAPI for nuclear staining.

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