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Anti-AMFR Antibody (CAB3731)

SKU:
CAB3731
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
$167 - $503
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Description

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Antibody Name:Anti-AMFR Antibody
Antibody SKU:CAB3731
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 384-643 of human AMFR (NP_001135.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:SKOV3, DU145
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 384-643 of human AMFR (NP_001135.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IADN NRVR EEHQ GENL DENL VPVA AAEG RPRL NQHN HFFH FDGS RIAS WLPS FSVE VMHT TNIL GITQ ASNS QLNA MAHQ IQEM FPQV PYHL VLQD LQLT RSVE ITTD NILE GRIQ VPFP TQRS DSIR PALN SPVE RPSS DQEE GETS AQTE RVPL DLSP RLEE TLDF GEVE VEPS EVED FEAR GSRF SKSA DERQ RMLV QRKD ELLQ QARK RFLN KSSE DDAA SESF LPSE GASS DPVT LRRR MLAA AAER RLQK QQTS
Gene ID:267
Uniprot:Q9UKV5
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:72kDa
Observed MW:72kDa
Synonyms:AMFR, GP78, RNF45
Background:This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins.
UniProt Protein Function:AMFR: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis. Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation. Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Ligase; Membrane protein, integral; EC 6.3.2.19; Endoplasmic reticulum; Ubiquitin conjugating system; Motility/polarity/chemotaxis; EC 6.3.2.-; Membrane protein, multi-pass; Receptor, misc.

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: endoplasmic reticulum membrane; growth cone; protein complex; cell soma; membrane; perinuclear region of cytoplasm; dendrite; integral to membrane; integral to endoplasmic reticulum membrane; nucleus

Molecular Function:protein binding; zinc ion binding; ubiquitin-protein ligase activity; receptor activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; ER-associated protein catabolic process; protein polyubiquitination; learning and/or memory; unfolded protein response; cell motility; signal transduction; protein oligomerization; aging

NCBI Summary:This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]
UniProt Code:Q9UKV5
NCBI GenInfo Identifier:113707
NCBI Gene ID:267
NCBI Accession:P26442.1
UniProt Secondary Accession:Q9UKV5,P26442, Q8IZ70,
UniProt Related Accession:Q9UKV5
Molecular Weight:323
NCBI Full Name:
NCBI Synonym Full Names:autocrine motility factor receptor
NCBI Official Symbol:AMFR  
NCBI Official Synonym Symbols:GP78; RNF45  
NCBI Protein Information:E3 ubiquitin-protein ligase AMFR
UniProt Protein Name:E3 ubiquitin-protein ligase AMFR
UniProt Synonym Protein Names:Autocrine motility factor receptor; AMF receptor; RING finger protein 45; gp78
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:AMFR  
UniProt Entry Name:AMFR_HUMAN
Anti-AMFR Antibody (CAB3731)Western blot analysis of extracts of various cell lines, using AMFR antibody (CAB3731) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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