Anti-ACY1 Antibody (CAB6351)

Antibody Name:	Anti-ACY1 Antibody
Antibody SKU:	CAB6351
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-408 of human ACY1 (NP_001185824.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, U-251MG, A-431, Jurkat, Mouse brain, Mouse liver, Mouse kidney, Rat kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-408 of human ACY1 (NP_001185824.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTSK GPEE EHPS VTLF RQYL RIRT VQPK PDYG AAVA FFEE TARQ LGLG CQKV EVAP GYVV TVLT WPGT NPTL SSIL LNSH TDVV PVFK EHWS HDPF EAFK DSEG YIYA RGAQ DMKC VSIQ YLEA VRRL KVEG HRFP RTIH MTFV PDEE VGGH QGME LFVQ RPEF HALR AGFA LDEG IANP TDAF TVFY SERS PWWV RVTS TGRP GHAS RFME DTAA EKLH KVVN SILA FREK EWQR LQSN PHLK EGSV TSVN LTKL EGGV AYNV IPAT MSAS FDFR VAPD VDFK AFEE QLQS WCQA AGEG VTLE FAQK WMHP QVTP TDDS NPWW AAFS RVCK DMNL TLEP EIMP AATD NRYI RAVG VPAL GFSP MNRT PVLL HDHD ERLH EAVF LRGV DIYT RLLP ALAS VPAL PSDS
Gene ID:	95
Uniprot:	Q03154
Cellular Location:	Cytoplasm
Calculated MW:	37kDa/38kDa/42kDa/45kDa
Observed MW:	46kDa

Synonyms:

ACY1, ACY-1, ACY1D, HEL-S-5

Background:

This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.

UniProt Protein Function:	ACY1: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. Belongs to the peptidase M20A family.
UniProt Protein Details:	Protein type:Hydrolase; EC 3.5.1.14; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: cytosol Molecular Function:metallopeptidase activity; metal ion binding; aminoacylase activity Biological Process: amino acid metabolic process; xenobiotic metabolic process; proteolysis Disease: Aminoacylase 1 Deficiency
NCBI Summary:	This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010]
UniProt Code:	Q03154
NCBI GenInfo Identifier:	461466
NCBI Gene ID:	95
NCBI Accession:	Q03154.1
UniProt Secondary Accession:	Q03154,C9J6I6, C9J9D8, C9JWD4,
UniProt Related Accession:	Q03154
Molecular Weight:	408
NCBI Full Name:	Aminoacylase-1
NCBI Synonym Full Names:	aminoacylase 1
NCBI Official Symbol:	ACY1
NCBI Official Synonym Symbols:	ACY-1; ACY1D; HEL-S-5
NCBI Protein Information:	aminoacylase-1; epididymis secretory protein Li 5; N-acyl-L-amino-acid amidohydrolase
UniProt Protein Name:	Aminoacylase-1
UniProt Synonym Protein Names:	N-acyl-L-amino-acid amidohydrolase
Protein Family:	Aminoacylase
UniProt Gene Name:	ACY1
UniProt Entry Name:	ACY1_HUMAN

	Western blot analysis of extracts of various cell lines, using ACY1 antibody (CAB6351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using ACY1 antibody (CAB6351). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-ACY1 Antibody (CAB13458)	Human Aminoacylase-1 (ACY1) ELISA Kit
ACY1 Antibody (PACO07596)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)