Anti-CIAPIN1 Antibody (CAB6336)

Antibody Name:	Anti-CIAPIN1 Antibody
Antibody SKU:	CAB6336
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human CIAPIN1 (NP_064709.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	SKOV3, 293T, HepG2, HeLa, MCF-7, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human CIAPIN1 (NP_064709.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADF GISA GQFV AVVW DKSS PVEA LKGL VDKL QALT GNEG RVSV ENIK QLLQ SAHK ESSF DIIL SGLV PGST TLHS AEIL AEIA RILR PGGC LFLK EPVE TAVD NNSK VKTA SKLC SALT LSGL VEVK ELQR EPLT PEEV QSVR EHLG HESD NLLF VQIT GKKP NFEV GSSR QLKL SITK KSSP SVKP AVDP AAAK LWTL SAND MEDD SMDL IDSD ELLD PEDL KKPD PASL RAAS CGEG KKRK ACKN CTCG LAEE LEKE KSRE QMSS QPKS ACGN CYLG DAFR CASC PYLG MPAF KPGE KVLL SDSN LHDA
Gene ID:	57019
Uniprot:	Q6FI81
Cellular Location:	Cytoplasm, Mitochondrion, Mitochondrion intermembrane space, Nucleus
Calculated MW:	11kDa/32kDa/33kDa
Observed MW:	39kDa

Synonyms:	CIAPIN1, Anamorsin, DRE2, PRO0915, anamorsin
Background:

UniProt Protein Function:	CIAPIN1: May be required for the maturation of extramitochondrial Fe/S proteins. Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells. Belongs to the anamorsin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis Chromosomal Location of Human Ortholog: 16q21 Cellular Component: cytoplasm; nucleolus; nucleoplasm Molecular Function:protein binding Biological Process: iron-sulfur cluster assembly; negative regulation of apoptosis
NCBI Summary:	CIAPIN1 is a cytokine-induced inhibitor of apoptosis with no relation to apoptosis regulatory molecules of the BCL2 (MIM 151430) or CASP (see MIM 147678) families. Expression of CIAPIN1 is dependent on growth factor stimulation (Shibayama et al., 2004 [PubMed 14970183]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q6FI81
NCBI GenInfo Identifier:	57012667
NCBI Gene ID:	57019
NCBI Accession:	Q6FI81.2
UniProt Secondary Accession:	Q6FI81,O75206, O75207, Q9H0W1, Q9P1L7, A8K8B6,
UniProt Related Accession:	Q6FI81
Molecular Weight:	32,213 Da
NCBI Full Name:	Anamorsin
NCBI Synonym Full Names:	cytokine induced apoptosis inhibitor 1
NCBI Official Symbol:	CIAPIN1
NCBI Official Synonym Symbols:	DRE2; PRO0915; Anamorsin
NCBI Protein Information:	anamorsin
UniProt Protein Name:	Anamorsin
UniProt Synonym Protein Names:	Cytokine-induced apoptosis inhibitor 1
Protein Family:	Anamorsin
UniProt Gene Name:	CIAPIN1
UniProt Entry Name:	CPIN1_HUMAN

	Western blot analysis of extracts of various cell lines, using CIAPIN1 antibody (CAB6336) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver damage using CIAPIN1 Antibody (CAB6336) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using CIAPIN1 Antibody (CAB6336) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using CIAPIN1 Antibody (CAB6336) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using CIAPIN1 antibody (CAB6336). Blue: DAPI for nuclear staining.

Antibodies

CIAPIN1 Antibody (PACO08435)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)