Anti-ANAPC10 Antibody (CAB8330)

Antibody Name:	Anti-ANAPC10 Antibody
Antibody SKU:	CAB8330
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human ANAPC10 (NP_055700.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, 293T, Mouse lung, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human ANAPC10 (NP_055700.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTTP NKTP PGAD PKQL ERTG TVRE IGSQ AVWS LSSC KPGF GVDQ LRDD NLET YWQS DGSQ PHLV NIQF RRKT TVKT LCIY ADYK SDES YTPS KISV RVGN NFHN LQEI RQLE LVEP SGWI HVPL TDNH KKPT RTFM IQIA VLAN HQNG RDTH MRQI KIYT PVEE SSIG KFPR CTTI DFMM YRSI R
Gene ID:	10393
Uniprot:	Q9UM13
Cellular Location:
Calculated MW:	21kDa
Observed MW:	26kDa

Synonyms:	ANAPC10, APC10, DOC1
Background:

UniProt Protein Function:	APC10: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC10 family.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 4q31 Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
NCBI Summary:	ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011]
UniProt Code:	Q9UM13
NCBI GenInfo Identifier:	119625457
NCBI Gene ID:	10393
NCBI Accession:	EAX05052
UniProt Secondary Accession:	Q9UM13,Q2V500, Q9UG51, Q9Y5R0, D3DNZ7,
UniProt Related Accession:	Q9UM13
Molecular Weight:	21,252 Da
NCBI Full Name:	anaphase promoting complex subunit 10, isoform CRA_b
NCBI Synonym Full Names:	anaphase promoting complex subunit 10
NCBI Official Symbol:	ANAPC10
NCBI Official Synonym Symbols:	DOC1; APC10
NCBI Protein Information:	anaphase-promoting complex subunit 10
UniProt Protein Name:	Anaphase-promoting complex subunit 10
UniProt Synonym Protein Names:	Cyclosome subunit 10
Protein Family:	Anaphase-promoting complex
UniProt Gene Name:	ANAPC10
UniProt Entry Name:	APC10_HUMAN

	Western blot analysis of extracts of various cell lines, using ANAPC10 antibody (CAB8330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human stomach using ANAPC10 antibody (CAB8330) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using ANAPC10 antibody (CAB8330) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)