The ANGPTL3 Monoclonal Antibody (CAB5225) is a high-quality antibody developed for reliable detection and analysis of target proteins. Developed using rabbit monoclonal technology, this antibody offers high specificity and sensitivity for detecting ANGPTL3 in human samples, making it ideal for use in various experimental settings.ANGPTL3, a member of the angiopoietin-like protein family, is known to regulate lipid levels in the bloodstream by inhibiting lipoprotein lipase activity. Dysregulation of ANGPTL3 has been linked to dyslipidemia, atherosclerosis, and other metabolic disorders, making it an attractive target for drug discovery and clinical research.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ANGPTL3 Monoclonal Antibody
SKU:
CAB5225
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1214
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ANL3, ANG-5, FHBL2, ANGPT5, ANGPTL3
Positive Sample:
A-375, 293T, Mouse liver, Rat liver
Cellular Localization:
Secreted.
Calculated MW:
54kDa
Observed MW:
62kDa
This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2.
Purification Method
Affinity purification
Gene ID
27329
RRID
AB_2863493
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ANGPTL3 Rabbit mAb (CAB5225) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ANGPTL3 Rabbit mAb (CAB5225) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Mouse liver using ANGPTL3 Rabbit mAb (CAB5225, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
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