The Ankh Polyclonal Antibody (CAB9881) is a valuable tool for researchers studying the Ankh protein, a key player in bone mineralization and homeostasis. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it an ideal choice for Western blot analysis.Ankh, also known as progressive ankylosis protein, is involved in the regulation of pyrophosphate levels in the body, which are essential for preventing mineralization of soft tissues. Dysregulation of Ankh function has been associated with various skeletal disorders, making it a target of interest in bone research.
By using the Ankh Polyclonal Antibody, researchers can gain insights into the expression and localization of the Ankh protein in different cell types and tissues. This information is crucial for understanding the mechanisms underlying bone metabolism and mineralization, with potential implications for the development of treatments for skeletal disorders.Overall, the Ankh Polyclonal Antibody is a valuable research tool for investigating the role of Ankh in bone biology and related conditions, with the potential to contribute to advancements in the field of skeletal research.
Product Name:
ANKH Rabbit Polyclonal Antibody
SKU:
CAB9881
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 210-320 of human ANKH (NP_473368.1).
This gene encodes a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals. Mutations in this gene have been associated with autosomal dominant craniometaphyseal dysplasia.
Purification Method:
Affinity purification
Gene ID:
56172
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ANKH Antibody (CAB9881) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
