Anti-ANKH Antibody (CAB9881)

Antibody Name:	Anti-ANKH Antibody
Antibody SKU:	CAB9881
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-320 of human ANKH (NP_473368.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	SH-SY5Y, OVCAR3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-320 of human ANKH (NP_473368.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LGYY KNIH DIIP DRSG PELG GDAT IRKM LSFW WPLA LILA TQRI SRPI VNLF VSRD LGGS SAAT EAVA ILTA TYPV GHMP YGWL TEIR AVYP AFDK NNPS NKLV STSN TVT
Gene ID:	56172
Uniprot:	Q9HCJ1
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	32kDa/54kDa
Observed MW:	54kDa

Synonyms:

ANKH, ANK, CCAL2, CMDJ, CPPDD, HANK, MANK

Background:

This gene encodes a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals. Mutations in this gene have been associated with autosomal dominant craniometaphyseal dysplasia.

UniProt Protein Function:	ANKH: Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter. Defects in ANKH are the cause of chondrocalcinosis 2 (CCAL2). Chondrocalcinosis is a common cause of joint pain and arthritis caused by calcium deposition in articular cartilage and the presence of calcium hypophosphate crystals in synovial fluid, cartilage and periarticular soft tissue. CCAL2 inheritance is autosomal dominant. Defects in ANKH are the cause of craniometaphyseal dysplasia Jackson type (CMDJ). CMDJ is a rare autosomal dominant skeletal disorder characterized by abnormal bone formation and mineralization in membranous as well as endochondral bones. Progressive thickening of the bones can cause narrowing of cranial foramina and can lead to severe visual and neurological impairment, such as facial palsy and deafness. Belongs to the ANKH family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 5p15.1 Cellular Component: integral to membrane; integral to plasma membrane; outer membrane; plasma membrane Molecular Function:inorganic diphosphate transmembrane transporter activity; inorganic phosphate transmembrane transporter activity Biological Process: regulation of bone mineralization; transmembrane transport Disease: Chondrocalcinosis 2; Craniometaphyseal Dysplasia, Autosomal Dominant
NCBI Summary:	This gene encodes a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals. Mutations in this gene have been associated with autosomal dominant craniometaphyseal dysplasia. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9HCJ1
NCBI GenInfo Identifier:	17366849
NCBI Gene ID:	56172
NCBI Accession:	Q9HCJ1.2
UniProt Secondary Accession:	Q9HCJ1,Q9NQW2, B2RCA7, B3KMG4, D3DTD4,
UniProt Related Accession:	Q9HCJ1
Molecular Weight:	32,259 Da
NCBI Full Name:	Progressive ankylosis protein homolog
NCBI Synonym Full Names:	ANKH inorganic pyrophosphate transport regulator
NCBI Official Symbol:	ANKH
NCBI Official Synonym Symbols:	ANK; CMDJ; HANK; MANK; CCAL2; CPPDD
NCBI Protein Information:	progressive ankylosis protein homolog
UniProt Protein Name:	Progressive ankylosis protein homolog
Protein Family:	Progressive ankylosis protein
UniProt Gene Name:	ANKH
UniProt Entry Name:	ANKH_HUMAN

	Western blot analysis of extracts of various cell lines, using ANKH Antibody (CAB9881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human prostate using ANKH antibody (CAB9881) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)