The alpha-Actin-1 (ATCA1) Monoclonal Antibody (CAB2280) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the ACTA1 protein, this antibody allows for detection and analysis in various cell types, making it ideal for research in muscle biology and related fields.ACTA1, also known as alpha-actin-1 or skeletal muscle actin, is essential for muscle function and is expressed predominantly in skeletal muscle.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
alpha-Actin-1 (ATCA1) Monoclonal Antibody
SKU:
CAB2280
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1914
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ITIG NERF RCPE TLFQ PSFI GMES AGIH ETTY NSIM KCDI DIRK DLYA NNVM SGGT TMYP GIAD RMQK EITA LAPS TMKI KIIA PPER KYSV WIGG SILA S
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause a variety of myopathies, including nemaline myopathy, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects with manifestations such as hypotonia.
Purification Method
Affinity purification
Gene ID
58
RRID
AB_2862988
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using α-Actin-1 (ACTA1) Rabbit mAb (CAB2280) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using α-Actin-1 (ACTA1) Rabbit mAb (CAB2280) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
