The HRP-conjugated β-Actin Rabbit mAb (CABC028) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, raised in rabbits and conjugated to HRP for enhanced detection capabilities, is highly specific to actin and is validated for use in a variety of applications, including Western blotting and immunofluorescence.Actin plays a critical role in a wide range of cellular processes, such as cell division, muscle contraction, and cell motility. By using the HRP Conjugated Actin Rabbit Monoclonal Antibody, researchers can accurately detect and analyze actin expression and localization in different cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Chicken, Zebrafish, Pig samples.
Product Name:
HRP-conjugated β-Actin Rabbit mAb
SKU:
CABC028
Size:
50μL, 100μL, 200μL
Reactivity:
Human, Mouse, Rat, Chicken, Zebrafish, Pig
Clone Number:
ARC5115-01
Conjugate:
HRP
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:50000-1:200000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRWS1, PS1TP5BP1, HRP-conjugated β-Actin
Positive Sample:
293T, HeLa, NIH/3T3, C2C12, C6, PC-12, Rat brain, Rat testis
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Purification Method
Affinity purification
Gene ID
60
RRID
AB_2769861
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HRP-conjugated β-Actin Rabbit mAb (CABC028) at 1:200000 dilution incubated overnight at 4℃. Secondary antibody: () at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
