The Biotin Antibody (CAB18848) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, is ideal for use in various immunological assays, such as ELISA, immunoprecipitation, and flow cytometry.The antibody binds specifically to biotin, allowing for accurate detection and quantification of biotinylated proteins in samples. Its high reactivity with biotin makes it a valuable tool for researchers studying protein biotinylation, protein-protein interactions, and signal transduction pathways involving biotinylated molecules.
This antibody is validated for use in WB applications and has demonstrated reactivity against Species independent samples.
Product Name:
Biotin Antibody
SKU:
CAB18848
Size:
20μL, 100μL
Reactivity:
Species independent
Immunogen:
Chemical compounds corresponding to Biotin.
Tested Applications:
WB
Recommended Dilution:
WB
,1:500 - 1:2000
Positive Sample:
Biotin-Goat-Mouse IgG
Observed MW:
25kDa(Lightchain),55kDa(Heavychain)
It is well know now that biotin plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth and development. Biotin is a water-soluble vitamin required by all organisms by virtue of its essential role in carboxylation reactions. Streptavidin and its homologs (together referred to as streptavidin) are widely used in molecular science owing to their highly selective and stable interaction with biotin.
Purification Method
Affinity purification
RRID
AB_2862477
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb (CAB18848) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 100 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
