Description
Biotin Monoclonal Antibody (CAB20684)
The Biotin Monoclonal Antibody (CAB20684) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with biotin and is validated for use in applications such as immunofluorescence and flow cytometry. By specifically binding to biotin molecules, the antibody enables efficient detection and analysis of biotinylated proteins in different experimental settings.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Other (Wide Range Predicted) samples.
| Product Name: | Biotin Monoclonal Antibody |
| SKU: | CAB20684 |
| Size: | 20μL, 100μL |
| Reactivity: | Other (Wide Range Predicted) |
| Clone Number: | ARC50695 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Positive Sample: | Biotin |
| Observed MW: | 25-55kDa |
Biotin is an organic heterobicyclic compound that consists of 2-oxohexahydro-1H-thieno[3,4-d]imidazole having a valeric acid substituent attached to the tetrahydrothiophene ring. The parent of the class of biotins. It has a role as a prosthetic group, a coenzyme, a nutraceutical, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a mouse metabolite, a cofactor and a fundamental metabolite. It is a member of biotins and a vitamin B7. It is a conjugate acid of a biotinate.
| Purification Method | Affinity purification |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates, using Biotin Rabbit mAb (CAB20684) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
