Western blot analysis of extracts of various cell lines, using Biotin Rabbit mAb (CAB22315) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Biotin Monoclonal Antibody (CAB22315) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal antibody technology, offers high specificity and sensitivity for detecting biotin in various biological samples, particularly in immunohistochemistry and ELISA applications. By targeting the biotin molecule, researchers can accurately visualize and quantify biotin levels in cells and tissues, providing valuable insights into biotin-related processes and functions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Other (Wide Range Predicted) samples.
Product Name:
Biotin Monoclonal Antibody
SKU:
CAB22315
Size:
20μL, 100μL
Reactivity:
Other (Wide Range Predicted)
Clone Number:
ARC50706
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Observed MW:
Refertofigures
Biotin is an organic heterobicyclic compound that consists of 2-oxohexahydro-1H-thieno[3,4-d]imidazole having a valeric acid substituent attached to the tetrahydrothiophene ring. The parent of the class of biotins. It has a role as a prosthetic group, a coenzyme, a nutraceutical, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a mouse metabolite, a cofactor and a fundamental metabolite. It is a member of biotins and a vitamin B7. It is a conjugate acid of a biotinate.
Purification Method
Affinity purification
Gene ID
CAS:58-85-5
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Biotin Rabbit mAb (CAB22315) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 100ng/50ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
