Anti-CoREST/RCOR1 Antibody (CAB3568)

Product Name:	CoREST/RCOR1 Rabbit mAb
Product Code:	CAB3568
Size:	20uL, 50uL, 100uL
Synonyms:	COREST, RCOR
Applications:	WB, IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human CoREST/RCOR1

Applications:	WB, IHC
Recommended Dilutions:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HepG2, C2C12

Immunogen:	A synthesized peptide derived from human CoREST/RCOR1
Purification Method:	Affinity purification
Storage:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	23186
Uniprot:	Q9UKL0
Cellular Location:	Nucleus
Calculated MW:	66kDa
Observed MW:	65kDa

UniProt Protein Function:	RCOR1: Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Belongs to the CoREST family.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 14q32.31 Cellular Component: nucleoplasm; nucleus; transcription factor complex; transcriptional repressor complex Molecular Function:histone deacetylase activity; protein binding; transcription corepressor activity; transcription factor binding Biological Process: blood coagulation; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor). [provided by RefSeq, Aug 2011]
UniProt Code:	Q9UKL0
NCBI GenInfo Identifier:	74762776
NCBI Gene ID:	23186
NCBI Accession:	Q9UKL0.1
UniProt Secondary Accession:	Q9UKL0,Q15044, Q6P2I9, Q86VG5,
UniProt Related Accession:	Q9UKL0
Molecular Weight:	53,028 Da
NCBI Full Name:	REST corepressor 1
NCBI Synonym Full Names:	REST corepressor 1
NCBI Official Symbol:	RCOR1
NCBI Official Synonym Symbols:	RCOR; COREST
NCBI Protein Information:	REST corepressor 1
UniProt Protein Name:	REST corepressor 1
UniProt Synonym Protein Names:	Protein CoREST
Protein Family:	REST corepressor
UniProt Gene Name:	RCOR1
UniProt Entry Name:	RCOR1_HUMAN

	Western blot analysis of extracts of various cell lines, using CoREST/RCOR1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
	Western blot analysis of extracts of C2C12 cells, using CoREST/RCOR1 Rabbit mAb at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded rat lung using CoREST/RCOR1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human appendix using CoREST/RCOR1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse brain using CoREST/RCOR1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol.
	Immunoprecipitation analysis of 300ug extracts of THP-1 cells using 3ug CoREST/RCOR1 antibody at a dilition of 1:1000.
	Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using CoREST/RCOR1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.