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DEK Rabbit Monoclonal Antibody (CAB0315)

SKU:
CAB0315
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
D6S231E
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:DEK Rabbit Monoclonal Antibody
SKU:CAB0315
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 51-200 of human DEK (P35659).
Sequence:SLIV EGKR EKKK VERL TMQV SSLQ REPF TIAQ GKGQ KLCE IERI HFFL SKKK TDEL RNLH KLLY NRPG TVSS LKKN VGQF SGFP FEKG SVQY KKKE EMLK KFRN AMLK SICE VLDL ERSG VNSE LVKR ILNF LMHP KPSG KPLP KSKK TC
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:D6S231E; DEK
Positive Sample:HeLa,HCT 116,K-562
Conjugate:Unconjugated
Cellular Localization:B-WICH complex, Contractile fiber, Nucleolus, Nucleoplasm, Nucleus
Calculated MW:43kDa
Observed MW:50kDa

This gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:7913
Clone Number:ARC1825
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DEK Rabbit mAb (CAB0315) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

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