The EIF4A1/EIF4A2/EIF4A3 Antibody (CAB20251) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot applications. The EIF4A family of proteins, including EIF4A1, EIF4A2, and EIF4A3, are essential for mRNA translation and play a critical role in protein synthesis. Dysregulation of EIF4A proteins has been implicated in various diseases, including cancer, making them an important target for research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EIF4A1/EIF4A2/EIF4A3 Antibody
SKU:
CAB20251
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Purification Method
Affinity purification
Gene ID
1973 1974 9775
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eIF4A Rabbit pAb (CAB20251) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using eIF4A Rabbit pAb (CAB20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using eIF4A Rabbit pAb (CAB20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
